Not known Factual Statements About flow cytometers
Not known Factual Statements About flow cytometers
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Data-enriched characterization of mobile populations. Morphological details from illustrations or photos can insert into the richness of flow cytometry details. For instance, the figure reveals an in any other case traditional apoptosis assay making use of Annexin V and PI, adding cell imaging to characterize cells in Each individual population to expose morphologically unique attributes. These insights could not have already been gained from multiplex staining on your own.
Whether you’re a newbie or a professional, take a look at our methods to progress your investigation and experience in flow cytometry.
Combine acoustic focusing engineering with superior-parameter Examination for versatile spectral unmixing and conventional workflows.
Discover new insights into your experimental info by visualizing populations working with mixed fluorescence and morphology information. Our program interface wizards enable downsampling, concatenation (merged information) and dimensionality applications that happen to be crafted into the software for an intuitive, streamlined knowledge analysis encounter.
"A picture just isn't fairly truly worth one thousand dots, nonetheless it really helps you to see photos. It is great to view illustrations or photos that correlate with staining. Such as, AnnexinV-constructive cells that are morphologically distinct than wholesome cells, or CD14+ monocytes that happen to be greater plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Here, a skilled consumer has gated singlets confidently. Following evaluating the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate contains more than four% aggregates.
Pair your Attune flow cytometer having an autosampler for greater workflow effectiveness, and increase robotic integration for optimum throughput.
Regular picture quality even at large throughput Acoustic focusing plus a significant-pace digicam Mix to impression these T cells continually at reduced or substantial flow premiums. Conveniently modify aim and digital camera configurations to meet experimental requirements.
The Attune NxT cytometer may be the trusted workhorse instrument your lab can count on for constant and trusted effectiveness. The Attune CytPix product incorporates a superior-speed brightfield camera and AI-driven graphic Assessment application, with the latest updates which includes device learning instruments and an intuitive user interface, enabling Improved data precision, precision, and quicker insights into sample biology.
Buyers can view function illustrations or photos in authentic-time to confirm sample quality, optimize protocols, and document the morphology of every mobile inhabitants discovered for Assessment. Imaging seize also Rewards from acoustic focusing, which helps you to placement the cells to ensure a pointy, centered image is acquired.
AI-pushed automatic picture Evaluation Together with the Attune Cytometric Software interprets celebration attributes into distinctive morphology parameters that can be combined with standard fluorescence and scatter parameters to expose hugely reproducible populace adjustments and unique subsets.
We Earlier demonstrated the strength of imaging Auto-T/Ramos cell interactions. Let’s have a look at just the populace of best desire, the double constructive gatherings, to learn more. We can easily now use extended morphological parameters (here, circularity and skewness of depth) to even more analyze the options of such populations and refine gating on these gatherings, increasing info robustness.
Built-in dimensionality reduction applications enhanced resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to substantiate anticipated inhabitants resolution in lysed complete human blood. Consumer can see events from Just about every cluster for visual affirmation of mobile phenotypes.
No clean/no lyse leukocyte Investigation with imaging. Cells were being obtained from 24-hour-previous blood by dilution in one mM EDTA ( Skilled UX/UI enables customers to train AI types for expanded apps. (A) The “Practice Product” aspect offers annotation equipment and real-time model teaching precision.
At the guts of each Attune Flow Cytometer lies our groundbreaking acoustic focusing technology, which utilizes sound waves to meticulously align cells throughout the flow mobile. This progressive strategy surpasses standard hydrodynamic techniques, leading to quicker processing speeds, Fantastic details quality, and greater sample throughput.
The Attune Xenith cytometer is often a higher-parameter spectral analyzer, supplying both equally spectral unmixing and standard flow abilities for in depth and versatile cell Assessment.
The Attune CytPix Flow Cytometer will allow customers to visualize samples in the course of acquisition, confirm the precision of gating methods, and support effects with reliable documentation for publication purposes.
Right here, we present that by gating procedures working with image-dependent quantitative parameters can distinguish interacting cells from coincident events to a lot more properly analyze interacting website cells.